Working as a multi-disciplinary collaboration, this H2020 project aims to combine the knowledge of leading researchers and pioneering industry specialists to hone the technology and expertise needed to develop ground-breaking ways of visualising living tissue. The genetic content of a cell determines its function. Genes are constantly switched on and off from the birth of the cell up until it dies. This highly dynamic process is regulated both by internal (genetic) and external (environmental) factors. In this H2020 funded project we develop a new technology to visualise the active genes in real time. In other words, we will be able to see when a gene is switched on and off in a living neuron.
Dr John S. Fossey (University of Birmingham, UK) conducts the synthetic chemistry part of the project.
In the heart of the project is a novel high-tech microscope that is able to scan a volume of tissue and imgae the alkyne tag.
The role of the Nanostructures and Applied Spectroscopy Group at Wigner RCP is to develop a tuneable stimulated Raman scattering (SRS)...
Observation and analysis of endogenous transcriptional processes in living and functioning organisms is not feasible today due to technical constraints. Therefore our understanding of the nuclear processes, and the options to diagnose or intervene at genetic level, is seriously constrained. Our proposal offers a cutting-edge technique to overcome this limitation. The aim is to visualize nuclear processes in intact brains in real-time, leading to: a new high-tech product development; prototyping of scientific equipment at the research institutes; and, in the long run, new tools to study and cure human diseases.
1) develop Stimulated Raman Spectroscopy (SRS)-based technologies to visualize endogenous transcription at single cell and single gene level;
2) develop tools for loading alkyne-tagged nucleosides and nucleotide analogues into neurons;
3) distinguish neuron types without labelling by SRS;
4) track transcription processes in intact brain with high temporal and spatial resolution;
5) study link between single gene function and behavior;
6) develop and commercialise a 4D-SRS microscope for broad range of applications.
The ambition of NEURAM is reflected in the wide spread of interdisciplinary expertise of the consortium members. The consortium brings together leading academic and industrial partners, each of which has a distinct role or skill in the project.
Complex projects cannot be carried out in a single lab as it require expertiese in various fields. In this porject we pulled together an international team from the field of chemistry, physics, neuroscience, nanobiology, genetics and interdisciplinary research management. This page briefly describes the expertiese of each participants and the role in the project with links to the individual labs.
A succesful summer school on Biophotonics (Photonics meets Biology) was organized with the Mesobrain H2020 project in Tarragona, Spain in September, 2017. All NEURAM consortium members participated with lectures.
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